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KMID : 1145120180160020243
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Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 2 p.243 ~ p.254
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Anti-wrinkle and Skin turnover Improvement Effects of Niacinamide-dipeptide Convergence
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Kim Ga-Yeon
Lee Seung-Je
Jeon Mi-Ji
Kim Bo-Min
Kim Guen-Tae
Kang Sang-Moon
Lee Kee-Young
Shin Eun-Jin
Kim Sang-Yong
Kim Young-Min
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Abstract
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Purpose: This study aimed to determine the anti-wrinkle and skin turnover improvement effects of niacinamide-dipeptide convergence (N-peptide).
Method: The following methods were employed to assess the anti-wrinkle and skin turnover improvement effects of N-peptide: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, chicken chorioallantoic membrane (CAM) assay, reverse transcription polymerase chain reaction (RT-PCR), and three-dimensional (3D) cell culture.
Results: The MTT assay revealed that the N-peptide does not exert a cytotoxic effect in human keratinocytes (HaCaT). Moreover, the scratch wound healing and CAM assays showed that the N-peptide induced migratory properties and angiogenesis, respectively, in HaCaT cells. The expression of matrix metalloproteinases (MMP)-1, -2, -3, and -9 was measured using RT-PCR to evaluate the anti-wrinkle effect of N-peptides. The findings demonstrated that N-peptides were responsible for reducing the expression of the aforementioned MMPs. In addition, using western blotting, N-peptides were identified to increase energy metabolism activity. Furthermore, 3D cell culture analysis indicated improvement in the skin turnover rate because of the N-peptides.
Conclusion: Taken together, these results suggest that N-peptides may be a source of cosmetic agents for the improvement of wrinkles and skin turnover.
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KEYWORD
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Niacin-dipeptide convergence, Functional cosmetic, Anti-wrinkle, Skin barrier, Skin turnover rate
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